The Microscopy and Imaging Suite is located at 101 Langley Hall. It is a shared facility equipped with state-of-the-art instruments for optical and electron microscopy and Flow Cytometry. We are open to all members at the University of Pittsburgh and outside users. We are happy to assist students and researchers with their projects in all aspects. Please contact Chaowei Shang for experimental design, equipment training, staff-operated image acquisition, and image analysis.
All users must be trained by facility staff to access the equipment.
Acknowledgements
Acknowledgement tracking is one method used by the Office of Research to prioritize internal funding requests.
Please acknowledge work done in the Microscopy and Imaging Suite in your publications with the following text:
Work performed in the University of Pittsburgh Dietrich School Microscopy and Imaging Suite (RRID:SCR_022084) and services and instruments used in this project were graciously supported, in part, by the University of Pittsburgh.
Microscopy Facility Rates
Equipment rates are current as of 3/1/2023.
| Peak/hour | Off-Peak/hour | Staff provide imaging | |
| Confocal | $35 | $25 | $75 |
| TEM | $35 | $25 | $75 |
| SEM | $25 | $20 | $75 |
| Flow Cytometer | $45 | $40 | NA |
Laboratory Instruments
| Instrument Type | Instrument | Facility |
| Biological Instrumentation | Attune Flow Cytometer | Microscopy and Imaging Suite |
| Microscopy and Imaging | HITACHI 7800 Transmission Electron Microscope | Microscopy and Imaging Suite |
| Microscopy and Imaging | JEOL JSM-6390LV Scanning Electron Microscope | Microscopy and Imaging Suite |
| Microscopy and Imaging | Leica TCS SP8 confocal microscope | Microscopy and Imaging Suite |
| Microscopy and Imaging | Leica Stereo Microscope | Microscopy and Imaging Suite |
| Microscopy and Imaging | Leica TCS SP5 Confocal and 2-Photon Microscope | Microscopy and Imaging Suite |
| Microscopy and Imaging | Nikon A1R-HD TIRF Confocal Microscope | Microscopy and Imaging Suite |
| Microscopy and Imaging | Olympus FV1000 Fluoview Confocal Microscope | Microscopy and Imaging Suite |
| Microscopy and Imaging | Olympus Abbelight Super Resolution Microscope-STORM | Microscopy and Imaging Suite |
Additional Equipment
- Image Analysis Workstation
- Ultra-microtomes (Glass & Diamond knives)
- Microtome
- Ultra-microtome knife maker
- Sputter coater (for SEM)
Equipment Usage
Follow these steps to use the equipment:
- Contact Chaowei Shang to discuss which microscope to use, experimental design, etc.
- Schedule a time for training → Register as a new user on the FOM scheduling system (a financial account is needed)
- Get trained
- For first-time use, please schedule a time during work hours just in case you need assistance
- You are now free to use the scope on your own (You can access the microscopes at any time)