Instrument: Leica TCS SP5 Confocal and 2-Photon Microscope

Type

Microscopy and Imaging

Facility

101H Langley Hall

Contact

Description

This is an advanced upright laser scanning confocal and 2-photon microscope. It incorporates a galvo scanner and a high-speed resonant scanner, as well as a wide range of laser lines. The two-photon system is built with Spectra-physics IR laser. This scope also features a programmable motorized stage, which can be used for montages and multiple sample time-lapse experiments.

Capabilities:

  1. 2D & 3D imaging
  2. Live and fixed samples (No environmental control)
  3. Live animals (mouse, rats, frog, etc.)
  4. FRAP
  5. Line scan
  6. Two-photon imaging
  7. Multi-position
  8. Tile scan /Large image stitching
  9. time-lapse imaging

Lasers(nm): 405; 476; 488; 493; 514; 561; 633

Scanners:

  1. Galvo scanner
  2. 1k x 1k resonant scanner

Detectors:

  1. Two fluorescent PMTs
  2. Two HyD detectors (Hybrid, high sensitivity)
  3. One Transmitted light PMT

Objectives

Name Magnification & NA Immersion Location
Leica 5x Dry Microscope
HC PL APO 10x   0.4NA  CS Dry Microscope
HC PL APO 40x   1.3NA  CS2 Oil Microscope
HCx PL APO 63x   1.4NA  CS2 Oil Microscope
HCX APO L63x 0.9NA Water (Dipping) Microscope
The 20x, 25x, and 100x objectives are all swappable from the Leica SP8.