Details
This laser scanning confocal is built upon a Nikon inverted microscope platform. It utilizes Nikon's adjustable speed high resolution galvano scanner and the new ultra-fast resonant scanner. For TIRF (total internal reflectance fluorescence) imaging, the microscope is equipted with Photometrics PRIME BSI. The microscope incorporates a Piezo stage for ultra-fast Z imaging, and an Tokaihit environmental control chamber for live-cell imaging. There are also inserts for imaging slides, dishes, and multiple well microplates.
Capabilities
- Fixed and live sample (With environmental control)
- Fluorescent 2D & 3D imaging
- Bright field/ Transmitted light imaging
- Line Scan
- FRAP & FRET
- TIRF
- High-throughput imaging
- Multi-point imaging
- Large image scan and stitching
- Time-lapse imaging
- Spectral Imaging
Lasers
405, 445, 488, 514, 561, 640
Scanners
Galvo scanner & 1k x 1k Resonant scanner
Emission
Filter tubes and Tunable Spectral Emission (Spectral detection)
Objectives
| Name | Magnification & NA | Immersion | Location |
| Plan Apo | 10x 0.45NA | Dry | Microscope |
| Plan Apo | 20x 0.75NA | Dry | Microscope |
| Plan Fluor | 40x 1.3NA | Oil | Microscope |
| Apo LWD | 40x 1.15NA | Water | Microscope |
| S Plan Fluor ELWD | 40x 0.6NA | Dry | Cabinet |
| Plan Apo | 40x 0.95NA | Dry | Cabinet |
| Plan Apo | 60x 1.4NA | Oil | Microscope |
| Apo TIRF | 100x 1.49NA | Oil | Microscope |
Type
Microscopy and Imaging
Facility
101G Langley Hall