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Leica TCS SP8 Confocal Microscope

SRSS laboratory equipment
Leica TCS SP8 Confocal Microscope

Details

The SP8 is a fully automated upright laser scanning confocal microscope that is capable of tunable emission spectral detection. It is equipped with a Galvano and a Resonant scanner that allows both high resolution and fast speed imaging. The system has a wide range of objectives ranging from 10x to 100x. The extra CLARITY optics (25x objective) enable unprecedented levels of in-depth optical sectioning of thick tissue.

Capabilities:

  1. 2D and 3D imaging (with 3D reconstruction feature)
  2. Live and fixed sample (No environmental control)
  3. FRAP
  4. Line scan
  5. Tile scan /Large image stitching
  6. Multi-position imaging

Lasers (nm): 405, 488, 514, 552, 638

Scanners:

  1. Traditional Galvo scan
  2. 1k x 1k Resonant scan

Detectors: 

  1. Two fluorescent PMTs
  2. Two HyD detectors (Faster and high sensitivity)
  3. One transmitted light PMT.
  4. Spectral Detection (Tunable emission wavelength)

Objectives:

Name Magnification & NA Immersion Location
HC PL APO 10x      0.4NA     CS2 Dry Microscope
HC PL APO 20x      0.75NA   CORR CS2 IMM (Water/Oil/Gly) Microscope
HCX APO L 20x   1.0NA  Water (Dipping) Cabinet
HCX IRAPO L 25x   0.95NA Water (Dipping) Microscope
HC FLUOTAR L 25x   1.0NA     motCORR VISIR IMM (Dipping) Cabinet
HC PL APO 40x      1.3NA     CS2 Oil Microscope
HCX PL APO 40x      0.85NA   CORR CS Dry Cabinet
HC PL APO  63x      1.4NA     CS2 Oil Microscope
HCX APO L 63x   0.9NA     U-V-I Water (Dipping) Microscope
HC PL APO 100x    1.44NA   CORR CS Oil Cabinet

Type

Microscopy and Imaging

Facility

Microscopy and Imaging Suite

101D Langley Hall