Details
This is an advanced upright laser scanning confocal and 2-photon microscope. It incorporates a galvo scanner and a high-speed resonant scanner, as well as a wide range of laser lines. The two-photon system is built with Spectra-physics IR laser. This scope also features a programmable motorized stage, which can be used for montages and multiple sample time-lapse experiments.
Capabilities:
- 2D & 3D imaging
- Live and fixed samples (No environmental control)
- Live animals (mouse, rats, frog, etc.)
- FRAP
- Line scan
- Two-photon imaging
- Multi-position
- Tile scan /Large image stitching
- time-lapse imaging
Lasers(nm): 405; 476; 488; 493; 514; 561; 633
Scanners:
- Galvo scanner
- 1k x 1k resonant scanner
Detectors:
- Two fluorescent PMTs
- Two HyD detectors (Hybrid, high sensitivity)
- One Transmitted light PMT
Objectives:
| Name | Magnification & NA | Immersion | Location |
| Leica | 5x | Dry | Microscope |
| HC PL APO | 10x 0.4NA CS | Dry | Microscope |
| HC PL APO | 40x 1.3NA CS2 | Oil | Microscope |
| HCx PL APO | 63x 1.4NA CS2 | Oil | Microscope |
| HCX APO | L63x 0.9NA | Water (Dipping) | Microscope |
| The 20x, 25x, and 100x objectives are all swappable from the Leica SP8. |
Type
Microscopy and Imaging
Facility
101H Langley Hall