Instrument: Nikon A1R-HD TIRF Confocal Microscope

Type

Microscopy and Imaging

Facility

101G Langley Hall

Contact

Description

This laser scanning confocal is built upon a Nikon inverted microscope platform. It utilizes Nikon's adjustable speed high resolution galvano scanner and the new ultra-fast resonant scanner. For TIRF (total internal reflectance fluorescence) imaging, the microscope is equipted with Photometrics PRIME BSI. The microscope incorporates a Piezo stage for ultra-fast Z imaging, and an Tokaihit environmental control chamber for live-cell imaging. There are also inserts for imaging slides, dishes, and multiple well microplates.


Capabilities

  1. Fixed and live sample (With environmental control)
  2. Fluorescent 2D & 3D imaging
  3. Bright field/ Transmitted light imaging
  4. Line Scan
  5. FRAP & FRET
  6. TIRF
  7. High-throughput imaging
  8. Multi-point imaging
  9. Large image scan and stitching
  10. Time-lapse imaging
  11. Spectral Imaging

Lasers

405, 445, 488, 514, 561, 640


Scanners

Galvo scanner & 1k x 1k Resonant scanner


Emission

Filter tubes and Tunable Spectral Emission (Spectral detection)


Objectives

Name Magnification & NA Immersion Location
Plan Apo 10x    0.45NA Dry Microscope
Plan Apo 20x    0.75NA Dry Microscope
Plan Fluor 40x    1.3NA Oil Microscope
Apo LWD 40x    1.15NA Water Microscope
S Plan Fluor ELWD 40x    0.6NA Dry Cabinet
Plan Apo 40x    0.95NA Dry Cabinet
Plan Apo  60x    1.4NA Oil Microscope
Apo TIRF 100x  1.49NA Oil Microscope